Abstract
A strain of soil bacteria was isolated by plate culture with soybean hemicellulose B, and was identified as Bacillus pumilus No.24. The hemicellulase was extracted from the supernatant fluid of the submerged culture, and fractionated on a CM-Cellulose column and on a Biogel P-60 column. The characteristics of the enzyme were as follows; optimum temperature was about 50°C, and optimum pH was 5.5. The enzyme was stable at 40°C. The enzymatic hydrolysate of soybean hemicellulase B was fractioned on a Sephadex G-15 column and its paper chromatogram showed to contain two kinds of spot. After extraction of those substances for acidic hydrolysis, arabinose and galactose were proved to be the constitutive sugars of both those substances.
