Automated Assay of Organic Acids related to the TCA-cycle in the Germinating Radish Seed

Abstract

Automated assay of organic acids related to the TCA-cycle in Kaiware radish seed (Rephanus sativus, Linn.) was investigated. Hitachi liquid chromatography was improved so as to be used as a silica-gel column with organic solvent. Standard mixture of eleven organic acids related to the TCA-cycle were determined with stable reproducibility and seven of eleven organic acids showed high coefficient of correlation of reproducibility of O.95 or higher.
Organic acids mixtures from different growth stage of the radish seed which was cultivated in the dark room at 20°C were carried out by this method. Twelve organic acids with one unknown were detected and most of them increased with growth except of lactic acid.