Studies on Biosynthesis of 7 S and 11 S Proteins, the Main Components of Soybean Protein

Part II. The Preparation of initial protein fraction by column chromatography and the some physicochemical properties

Abstract

The initial protein was prepared from the defatted meal of unmatured soybean seed with urea by the column chromatography on DEAE-Sephadex A-50 and Sephadex G-150. The prepared protein showed a single peak (S₂₀, W=apporoximately 1.2) on the ultracentrifugal pattern and a single band on the starch gel electrophoretical pattern with urea. Also the molecular weight was estimated as 23, 000 from gel filtration with 8M urea on Sepharose 6B.
Little difference was found between the initial protein and 7S globulin from the matured seeds about amino acid composition, N-terminal amino acid composition and isoelectric point (apporoximately 5.10) by the method of isoelectrofocusing.