Studies on the Water-Insoluble Naringinase

Part I. Preparation of the water-insoluble naringinase with DEAE-cellulose

Abstract

The water-insoluble naringinase was obtained by linking the native naringinase to DEAEcellulose at pH6.0 with little loss in activity. This insoluble naringinase was compared with the native naringinase to obtain following results,
(1) The optimum pH(4.5) of the insoluble naringinase was not changed but the activity was strengthened at acid pH.
(2) The optimum temperature was shifted from 55°C to 50°C, while the peak of the temperature-activity curve was broader than the native.
(3) The heat stability was strengthened, especially at 60°C.
(4) The preservability was improved very much.