Studies on the Preparation of Immobilized Enzymes by Radiopolymerization
Part VIII. On the bead shaped immobilized invertase
1976 Volume 23 Issue 12 Pages 588-593
Details
1976 Volume 23 Issue 12 Pages 588-593
Invertase was immobilized in an bead shaped polymer(0.5-1mm diameter)by radiocopolyme-rization of some synthetic monomers at the frozen temperature. Some of the basic characteristics were investigated and enzyme column was tested.
1) Immobilized invertase preparations having high retained activities(60-90%)were prepared by polymerizing some monomers without acrylamide.
2) The optimum reaction temperature was about 65°C being 5°C higher than that of the native invertase.
3) Heat stability was greatly improved. After heating at 70°C for 12min, immobilized invertase hold sti11 70% of the original activity but native one had 40%.
4) Immobilized invertase was more resistant to aniline, urea and Hg++ but on the contrary, more sensitive to SDS, guanine and Cu++.
5) The Km value was 2.45×10-2 mole and 1.75×10-2 mole for immobilized and native enzyme respectively.
6) The activation energy was increased by 0.9Kcal/mole after immobilization.
7) When used as immobilized enzyme column, it was found to be more advantageous to increase the concentration of substrate and enzyme in the entrapping polymer.
8) 30% sucrose solution was continously charged in to the column at the flow rate of SV 1. The substrate was completely decomposed up to 28 or 50 days.
9) There were no big differences on the basic characteristics between bead shaped and membranous immobilized invertase preparations except for the behavious to some inhibitors.
