Studies on the Preparation of Immobilized Enzymes by Radiopolymerization

Part VI. Hydrolysis of sucrose solution by membranous immobilized invertase

Abstract

An investigation on the hydrolysis of sucrose solution by membranous immobilized invertase was conducted and the following results were obtained.
1) Sucrose solution(10 to 40% w/w)were decomposed completely by the immobilized invertase in a small reactor.
2) The immobilized invertase was repeatedly used in a reactor to decompose 10% sucrose sulution and there was no significant change in the decomposition pattern of substrate even after 30 times usage.
3) 30% sucrose solution was decomposed completely by the immobilized invertase column at the flow rate of SV3 to SV4.
4) The enzyme column was continuously operated and 30% sucrose solution was completely hydrolyzed up to 47 days at the flow rate of SV1.