Quantitative Analysis of Phytoglycolipids in Defatted Soybeans

Abstract

Extraction of phytoglycolipid(PGL)with a 70% ethanol solution under reflux was the most effective condition in the determination of PGL in defatted soybean meals. The extract was dialyzed against water and washed by Folch's method. The PGL remained in the dialysis tube and in the lower phase of Folch's washing. After alkaline hydrolysis, the PGL was determined on the basis of a phosphorus or a sugar analysis in the PGL spot on a thin-layer with silica gel by modified component of chloroform-methanol-conc. ammonium hydroxide-water(10:7.5:1.5:1.5). The PGL content of defatted soybean meals were 0, 075-0.080%.