A Simple Method for Preparation of Cellooligosaccharides

Abstract

Cotton cellulose powder (5g) was dissolved in 20ml solution of conc. H2SO4-conc. HCl (1:1) and then 60ml of conc. HCl was added, and it was hydrolyzed partially at 20°C for 5hr. The produced cellooligosaccharides were recovered by cold acetone fractionation from 70 to 95%, and the yield was about 250mg in tatal. The cellooligosaccharides were separated to G1, G2, G3, G4 and G5 by paper chromatography with n-butanol-pyridine-water (4:4:1) and the elution profile of the cellooligosaccharides by Bio-Gel P-4→P-2→P-2 series column showed a good separation of G1-G8. These results show that the cellooligosaccharides prepared by this method can be used for analytical standards of paper chromatography and filtration.