Identification of Mugi-Miso by Urea Polyacrylamide Gel Electrophoresis

Abstract

The densitometric protein patterns of the proteins in "Mugi-miso (barley-miso) were investigated by urea polyacrylamide gel electrophoresis. The proteins of soybean, rice and barley were identified by this method. Furthermore, "Mugi-miso" (barley-miso) made from soybean and barley could be discriminated from rice blended "Mugi-miso" (barley-miso). The height of barley protein band was nearly proportional to the barley content in "Mugi-miso" (barley-miso). Nine samples of the commercial "Mugi-miso" (barley-miso) were examined to detect their raw materials, and their mixing ratios. The ratio was 1 to 3 parts of barley to 1 part of soybean.