Mutants of Saccharomyces rouxii Incapable of Oxidizing Linoleic Acid

Abstract

of miso, mutants incapable of oxidizing linoleic acid were isolated by treatment with N-methyl-nitro-N-nitrosoguanidine and the properties of the mutants were examined in comparison with those of their parents. i)The results of the effects of pH and temperature on the growth of 35 strains of the mutants showed most of them to behave similarly to their parents. ii)The mutants incapable of oxidizing linoleic acid could still oxidize glucose, fructose and ethanol, indicating that they lack specific properties for the oxidation of linoleic acid. iii)By gas-liquid chromatography, 24 strains of the mutants showed different patterns in their composition of fatty acids from those of their parents. ix)Among 35 strains of the mutants, 20 strains showed lower activity in acyl-CoA synthetase than their parents. v)From the result of examination of respiratory deficient yeasts, 12 strains of the mutants seemed to have received some changes in their respiration, not observed in normal strains.