Analysis of Nucleic Acid Related Substances by Cation Exchange Chromatography

Abstract

Nucleic acid related substances were separated by cation exchange chromatography. The analysis was carried out using a column of φ9mm×400mm packed with Hitachi Model 2613 cation exchanger, by the stepwise eluting method. GMP, CMP, AMP, Urd, Ura, Thy, Ino, and Xan could be separated by 0.05 M monopotassium phosphate-phosphoric acid (pH 2.9) as the first eluting solution, Hyp. and Guo. by 0.4M monopotassium phosphate-phosphoric acid (pH 3.7) as the second eluting solution, and Ado, Cyd, Gua, Ade, and Cyo by 0.4M monopotassium phosphate-potassium hydroxide (pH 5.4) as the third eluting solution. The Nucleic acid related substances in leafy vegetables (Cabbage, Chinese cabbage, Head lettuce) were determined by this method. The trace amounts of Ade, Gua, Ado, Guo, and Cyd were observed in these samples.