Determination of a Small Amounts of Saccharin in Milk Beverages and Fermented Milk Beverages by Gas Chromatography

Abstract

A small amount of saccharin in milk beverages and fermented milk beverages was determined by gas chromatography. The results obtained were as follows: Saccharin was extracted by dialysis using cellulose tube and then methylated by diazomethane. The detecter of the gas chromatograph should be changed by the amount of saccharin which was analysed. FPD and ECD were suitable for the detection of μg of saccharin, and FID was suitable for mg of saccharin. The recovery rate was 80-100% in each case. It was revealed that this method was applicable to milk beverages and fermented milk beverages containing interfering substances.