Histological View by the Application of Adhesive Tape on Germinating Rice Seed

Studies on Raw Starch-Degrading Enzyme (Amylase) during the Germination of Rice Seed Part III

Abstract

We studied histological view, using an adhesive tape, in a germinating rice seed. To get clear figures of starch granules and starch tissues, we compared two adhesive tapes, Cellotape (Nichiban, Inc., Tokyo) and Scotch Brand Tapes (3M Philippines, Inc.). The adhesive tape was contacted to the smooth surface of a germinating rice seed to copy bared starch granules on it. The smooth surface was made by a microtome. In this comparison the Scotch Brand Tapes showed excellent quality. Starch tissues in the endosperm were eroded from the part attached to the epithelium of scutellum, and in the scutellum starch granules were accumulated. The starch granules in the endosperm were angular in shape, but at the partially eroded starch tissues in the endosperm, the starch granules became roundish and smaller.