Simultaneous Determination of 5'-IMP and 5'-GMP in Foods by Isotachoelectrophoresis

Abstract

Ion exchange chromatography and paper electrophoresis are usually used for the analyses of 5'-IMP and 5'-GMP in foods. However, these methods are complicated owing to the necessity of pre-treatment and concentration. Therefore, the possibility of isotachoelectrophoresis for the determination of the compounds was studied. For the leading electrolyte, 0.02M HCl solution (pH 2.35), for the terminal electrolyte, 0.01M n-caproic acid, and for the spacer ion, 1000ppm citric, lactic and α-ketoglutaric acids were used. By this procedure, the simultaneous determination of 5'-IMP and 5'-GMP was accomplished without any pre-treatment. The pricision (coefficient of variation) for 5'-IMP and 5'-GMP were 5.6% and 6.7%, respectively. The limit of determination for these two compounds was as low as 0.05μg, respectively.