1984 Volume 31 Issue 10 Pages 665-668
Contents of ascorbic acid (AsA), dehydro AsA and total AsA in 20 different kinds of fruit and vegetable tissues were determined by difference spectral method (DS) and 2, 4-dinitrophenylhydrazine method (DNP). AsA value obtained by DS was compatible with one by DNP, but values of total AsA and dehydro AsA by DS were lower than those by DNP. As to total AsA, the ratio of the value estimated by DNP to one by DS nearly agreed the ratio of the value estimated by DNP to "true value", which was obtained by the thin layer chromatography of osazone produced by coupling 2, 4-dinitrophenylhydrazine with dehydro AsA, reported by FUJITA et al. Consequently, it is likely that total AsA value determined by DS corresponds to real value of total AsA.
