Lipids in Cap and Stalk of Shiitake Mushroom

Studies on the Lipids of Shiitake Part I

Abstract

Three fractions-neutral lipids, glycolipids and phospholipids were obtained from total lipids in cap and stalk of "Koshin" (Shiitake, Lentinus edodes, with expanded cap), and their lipid compositions and fatty acid compositions were studied by means of silic acid chromatography, thin-layer chromatography, thinchrography and gas-liquid chromatography. The following results were obtained: (1) The total lipid content in the cap and stalk were 0.50g (4.58% in dry matter) and 0.45g (2.65% in dry matter), respectively. (2) Reviewing the composition of each lipid fraction in total lipids, it was found that the contents of neutral lipids (46.7-48.8%) and phospholipids (42.0-43.8%) were high, while that of glycolipids (9.2-9.5%) was low. (3) The main lipid in neutral lipids was triacylglycerol (58.0-58.5%) followed by sterol ester (16.9-17.4%), sterol (15.6-16.4%), diacylglycerol (5.8-6.0%) and monoacylglycerol (1.1-1.3%). (4) The main lipid in phospholipids was phosphatidyl ethanolamine (58.3-60.2%) followed by phos hatidyl choline (17.3-19.5%), cardiolipin (12.8-14.0%) and lyso-phosphatidyl choline (4.8-6.6%). (5) Fatty acid composition was not significantly different among total lipids, neutral lipids, glycolipids and phospholipids contained in cap and stalk. The main fatty acid was C 18:2 followed by C 16:0 and C 18:1 acids.