Identification of Acetic Acid Bacteria Isolated from Vinegar Mash

Studies on the Substances to Stimulate Acetic acid Fermentation Part VIII

Abstract

Acetobacter strain No. 2 and Acetobacter strain M-1 were non-motile, gram-negative rods and had the Q-9 (Q-8) system. They oxidized both acetate and lactate, and produced acetic acid from ethanol, however, did not produced dihydroxyacetone from glycerol and water soluble brown pigments. Growth on Hoyer-Frateur medium, the formation of thick pellicle or the formation of ferric chloride-positive substance in glucose and fructose broth were not observed. Acetobacter strain M-1 had the ability to grow on glutamate agar, and to produce acids from L-arabinose and D-galactose, whereas Acetobacter strain No. 2 did not have such properties. From these results, Acetobacter strain No. 2 and Acetobacter strain M-1 were identified as Acetobacter pasteurianus.