Simple Method for the Simultaneous Determination of Trigonelline and Caffeine in Coffee

Abstract

A simple method is reported for the simultaneous determination of trigonelline and caffeine in coffee. Trigonelline and caffeine are separated fully by Sephadex G 15 column (1.65×40cm). Polyamide adsorbs the almost polyphenolics which influence the ultraviolet absorption spectrophotometry. Chromatography is carried out with distilled water on the single column packed with 3.2g of polyamide placed on Sephadex G 15. The eluate is monitored at 270 and 300nm. The quantification is achived from the difference between the these absorbances of two peaks correspondentto trigonelline and caffeine on the elution chromatogram.