Isolation and Identification of 1-Kestose Produced from Sucrose by Fructose-Transferring Enzyme of Penicillium oxalicum

Abstract

One of the oligosaccharides produced from sucrose by fructose-transferring enzyme from Penicillium oxalicum was identified. This oligosaccharide was isolated by column chromatographies on charcoal-celite (1:1) and Sephadex G-15. The saccharide was identified as 1-kestose by acid hydrolysis, enzyme hydrolysis, gas-liquid chromatography of partialy methylated derivatives, mass spectrometry of trimethylsilylated derivative, and ¹³C-NMR spectroscopy.