Simultaneous Quantitative Determination of Allyl Isothiocyanate aud β-Phenethyl Isothiocyanate by Gas Chromatography Equipped with FPD

Abstract

Simultaneous quantitative determination of allyl isothiocyanate and β-phenethyl isothiocyanate by gas chromatography equipped with FPD was investigated. Column was packed with 5% PEG 20M/Chamelite CS. Internal standards used were thionaphthene and phenyl isothiocyanate, and n-hexane was used as a solvent. Relations of both y=ax+b and y=cx^d were recognized between the concentration of allyl isothiocyanate (x) or β-phenethyl isothiocyanate (x) and ratio of peak area of allyl isothiocyanate or β-phenethyl isothiocyanate vs. that of an internal standard (y). Values of a, b, c and d are constant in equations. Contents of each isothiocyanate in n-hexane extract of horseradish flour hydrolysate were determined using above both equations, and were nearly equal. Values calculated with thionaphthene as standard were nearly equal to those with phenyl isothiocyanate as well.