Determination of Ascorbic Acid Kineticaly by Using Ascorbate Oxidase

Abstract

The kinetic determination of ascorbic acid was investigated by using ascorbate oxidase. The initial rate, υ, for 0.1mM ascorbic acid was obtained with ascorbate oxidase on pH 5.6 at 25°C, which was different from those obtained with other reductones (erythorbic acid, reductic acid and triose reductone). Ascorbic acid can be determined kineticaly even in the presence of erythorbic acid, glucose, fructose, sucrose, sodium Lglutamate, glycine, KCI, CaCl₂, FeCl₃, MgCl₂ and NaCl. The sensitivity is estimated to be 0.03mM on pH 5.6 at 25°C.