Protective Effect of Dietary Fiber Prepared from Pulp Refuse after Extraction of Juice from Citrus Fruits against Amaranth Toxicity

Abstract

The pulp refuse of citrus fruits was produced as a by-product from their juice e xtraction process. To increase in their new uses, citrus dietary fiber (CDF) was prepared by washing them in hot water repeatedly. Four kinds of dietbry fiber (DF), which were CP (cellulose powder from cotton cellulose), WF (Avicel RC-N 81 from wood) and PF (Fiber P-1 f rom potatoes) on the market and CDF, were examined on their physical and chemical properties and on their preventive effects against amaranth toxicity caused by feeding of a diet containing 5% amaranth. The diets used for rats in this experiment were as follows. They were B (basal diet), A (B diet+5% amaranth), A1 CDF, A3 CDF, A5 CDF, A10 CDF, A3 CP, A5 CP, A10 CP, A5 WF (A diet+5% WF) and A5 PF (A diet+5% PF) diet. A1 CDF, A3 CDF, A5 CDF and A10 CDF diet were made by addition of 1, 3, 5 and 10% CDF to A diet and A3 CP, A5 CP and A10 CP diet were similarly prepared by additi on of 3, 5 and 10% CP to A diet. (1) Both WHC (water holding capacity) and SV (settling volume in water) values of four samples became larger in order CDF, PF, CP and WF. There was high correlation (Y=0.96 x-1.66, r=0.98) between WHC and SV of each sample. Their chemical compositions were rich in cellulose and pectic substances and poor in hemi chemical compositions were rich in cellulose and pectic substances and poor in hemi reduction in the growth rate of rats. The rats fed on A1 CDF, A3 CDF, A3 CP and A5 CP diet were not able to gain satisfactorily their body weight by diarrhea. The rats fed on A5 CDF diet were considerably restored to gain their body weight without diarrhea. The rats fed on A10 CDF diet grew up normaly as same as the rats fed on B diet. The rats fed on A10 CP diet grew up next to the rats fed on A5 CDF diet. The preventive effect of CDF against amaranth toxicity was recognized at the additional level of 5% CDF to A diet. (3) The rats fed on A5 CDF diet grew up next to the rats fed on B diet and the rats fed on A5 PF diet next to A5 CDF rats and they were suffered from a little amaranth tox icity in comparison with the rats fed on B diet. The rats fed on A5 CP or A5 WF diet were unable to increase satisfactorily their body weight by diarrhea caused by amaranth toxicity.