Protective Effect of Liver Extract on Acetic Acid Inhibitionagainst Debaryomyces hansenii

Studies on the Antimicrobial Mechanism of Acetic Acid Part I

Abstract

The growth inhibitory action of acetic acid and the protective effect of a liverextract onits inhibition against Debaryomyces hansenii were investigated to estimate the antimicrobialmechanism of acetic acid.(1) In a casamino acid yeast extract medium containing aceticacid (pH 5.0), the growth of yeast was inhibited with an increase of acid concentration, andbactericidal effect was recognized above 3.0% of acetic acid.(2) In the casaminacid yeastextract medium, the growth of resting cell treated in phosphate buffer solution (pH 5.0)which contained less than 4.0% of acetic acid for 3h at 30°C was the same level as that ofnontreated case.(3) The liver extract stimulated the growth of yeast in the casamino acidmino acidinhibitionby acetic acid in the same medium containing 1-4% of acetic acid. Moreover, theliverextract exhibited not only relief action on growth inhibition by acetic acid, but also recoveryaction from acid inhibition. (4) The protective substance for acetic acid inhibition which iscontained in the liver extract was estimated to be a compound that exhibit maximumabsorption near 260nm and comparatively low molecular weight.