Production of 4'-galactosyllactose by Mixed Cells ofCryptococcus laurentii and Baker's Yeast

Abstract

Glucose was released as a byproduct when 4'-galactosyllactose (4'-GL) was producedfrom lactose by resting or immobilized cells of Cryptococcus laurentii OKN-4. Fordigestion of this glucose, cells of Saccharomyces such as Baker's yeast were added in areaction mixture containing Ca-alginate immobilized cells of Cr. laurentii and lactose. As aresult, no glucose was observed in the reaction mixture, and the 4'-GL production rate withaddition of Baker's yeast was enhanced with little other oligosaccharides. In this reactionsystem, the operation pH was 3-7, the optimum temperature was 40°C and the repeatedtch reaction was possible. The cell quantity (at dry cell weight) of Baker's yeast requiredfor the reaction was 0.2 times as much as Cryptococcus