Studies on Production of Xylosylfructoside by Levansucrase

Abstract

Bacillus subtilis var. saccharolyticus was cultivated in a 500-liter fermentor c ontaining 200 liter of a medium of 5% sucrose, 5% corn steep liquor, 0.6% (NH₄)₂ HPO₄ and 0.02% KCl at 30°C for 16-24hours. Forty times as much levansucrase activity as in static culture was obtained in this culture broth. Invertase (sucrose hydrolyzing activity) was con taminated in the culture broth, but this activity was depressed by carring out the enzyme act ion at pH 5.0. Twenty liter of the enzyme solution (30 000 units) was incubated with the mixtur e of 10kg sucrose and 10kg xylose at pH 5.0 at 50°C for 20hours. From the reaction mixture, 5.7kg of xylosylfructoside was obtained after carbon column chromatography.