The Proximate Components, Free and Proteinbound Amino Acids in Protein and 5'-GMP in Fruit-Bodies of Lentinus edodes Singer (Shiitake Mushroom) Grown on Artificial Bed-blocks

Abstract

free and protein-bound amino acids and extractable 5'-GMP in fruit-bodies of Shiitake Mushroom (Lentinus edodes Sing.) grown on artificial bed-blocks examined. (1) The crude protein content was 25.9%and the carbohydrate was 67.0%. (2) The total free amino acid content was 1665mg/100g dry weight. In free amino acids component, glutamic acid was the dominant and was 385.9mg/100g dry weight. Free amino acids content per dry weight decreased slightly with the growth of fruit-body. (3) Among the protein-bound amino acids, glutamic acid was the major compound, while methionine and cysteine were minor ones. (4) The amount of 5'-GMP extracted from whole of matured fruit-bodies was 312.8mg/100g dry weight, and that in the cap of fruit-bodies was amounted to 2.6 times compared with the stem.