1990 Volume 37 Issue 8 Pages 594-601
Bovine plasma proteins were fractionated by the COHN's method, which has been used world-wide to produce plasma fractions. The separation of plasma into fractions concentrates most of fibrinogen in, Fr-I, of globulin in Fr-II+III, and of albumin in Fr-V. The interaction among myosin B, whole plasma, Fr-I, Fr-II+III and Fr-V was examined by measuring the changes in turbidity of a solution and SDS-PAGE. When the time course of turbidity was measured at pH 6.0 in the presence of 500mM NaCl by heating at 1°C/min from 30°C to 100°C, the turbidities of plasma, Fr-1, Fr-II+III and Fr-V were sharply increased from 73°Cto 82°C, from53°C to 63°C, from 70°C to 76°C and from88°C to 96°C, respectively. SDS-PAGE showed that plasma proteins were able to bind to myosin B, especially to myosin: and actin. The interaction between myosin B and fibrinogen was stronger than those between myosin B and globulin or myosin B and albumin.
