Effects of Mineral Salts, Vitamins, Nucleic Acid Relating Substances and Phytohormones on the Vegetative Growth of Kuritake Mushroom (Naematoloma sublateritium)

Abstract

Effects of mineral salts, vitamins, nucleic acid relating substances and phytohormones on the vegetative growth of kuritake mushroom [Naematoloma sublateritium (Kr.) Karst.] were investigated using a liquid culture medium. (1) The vegetative mycelium did not grow in the absence of KH₂PO₄ and MgSO₄·7H₂O. The vegetative mycelium yield was severely decreased by the omission of ZnSO₄·7H₂O. The other mineral salts [CaCl₂·2H₂O, FeSO₄·7H₂O, CuSO₄·5H₂O, MnSO₄·5H₂O, (NH₄)₆Mo₇O₂₄·H₂O] did not show any special effect for the vegetative growth. (2) The vegetative mycelium required thiamine for the growth. Each of the other vitamins (nicotinamide, riboflavin, pantothenic acid, pyridoxine, folic acid, cyanocobalamin, biotin, inositol) affected the vegetative growth. (3) Nucleic acid bases (adenine, guanine, cytosine, thymine, uracil, orotic acid), nucleosides (adenosine, guanosine, cytidine, inosine, uridine, thymidine) and nucleotides (adenylic acid, guanylic acid, cytidylic acid, uridylic acid) were effective for the vegetative growth. (4) The vegetative mycelium yield was increased in the basal medium containing 1mg/l of IAA (indole-3-acetic acid), NAA (1-naphthylacetic acid) and GA3 (gibberellic acid), but the vegetative mycelium yield was decreased in the basal medium containing 10mg/l of IAA and NAA.