Determination of Pantothenic Acid in Infant Formulas by High Performance Liquid Chromatography

Abstract

A high performance liquid chromatographic (HPLC) method was developed for the determination of pantothenic acid in infant formulas. The clean-up procedure was as follows; sample was dissolved in water and adjusted pH to 4.6 With HCl to precipitate protein and to extract pantothenic acid. The extract was purified on Chem Elute column and Bond Elute CH column. Pantothenic acid was separated on a Inertsil ODS-2 column with accetonitrile/20mM phosphate buffer (pH 3.0) [7+93] as a mobile phase. An UV detector was used for the detection of pantothenic acid and the wavelength was set at 200nm. The recoveries of added pantothenic acid were 92.0%-98.6%. The repeatability of this method was 3.3% as coefficient of variation. Twelve commercially available samples were analyzed by this method. The results almost agreed with those of microbiological method.