Identification and Composition of Low Molecular Weight Carbohydrates in Commercial Soybean Oligosaccharide Syrup

Abstract

Low molecular weight carbohydrates have been preparatively isolated from the soybean oligosaccharide syrup by high performance liquid chromatography using two column systems. Ten carbohydrates including six galacto-oligosaccharides and one cyclitol were clearly identified by ¹³C-NMR (500MHz). Composition of carbohydrates was estimated by comparing peak area of the HPLC peaks: stachyose (21.9%), raffinose (5.8%), manninotriose (5.4%), melibiose (1.1%), galactopinitol A (2.6%), galactopinitol B (2.4%), sucrose (34.6%), glucose (7.2%), fructose (8.3%) and pinitol (5.5%). The content of the growth activator of bifidobacteria (stachyose, raffinose, manninotriose and melibiose) was about 34%.