NIPPON SHOKUHIN KOGYO GAKKAISHI
Print ISSN : 0029-0394
Simple Method for Simultaneous Determination of Chlorophyll and Carotenoids in Tomato Fruit
Masayasu NAGATAIchiji YAMASHITA
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1992 Volume 39 Issue 10 Pages 925-928

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Abstract

The contents of chlorophyll and carotenoid in tomato fruits have been analyzed respectively by different methods in a conventional way. We studied simple method for simultaneous determination of the pigments in tomato. All pigments in sample were extracted with acetone-hexane (4:6) at once, then optical density of the supernatant were measured at 663nm, 645nm, 505nm and 453 nm by spectrophotometer at the same time. From these values, the content of chlorophyll a, b, lycopene and β-carotene could be estimated using our proposed equations. Tomatoes of different ripening stage were analyzed by our method. Also the same samples were analyzed by conventional methods. The results by our method were similar to those of chlorophyll contents by MACKINNEY'S method and of lycopene contents by KIMURA'S method. It confirmed the availability of our method.

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© Japanese Society for Food Science and Technology
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