Effect of Additives on Chlorophyllase from Goosefoot, Chenopodium alblum L.

Abstract

Effect of α-tocopherol, lecithin, and. menadione on chlorophyllase from goosefoot was investigated. The concentration of additives in a reaction mixture was 10 and 20mM. α-Tocophopherol which had phytyl side chain showed little effect on chlorophyllase activity. Lecithin which was an ampholytic surfactant accelerated the enzyme activity. Activity of the enzyme with lecithin was twice as much as that without lecithin. Menadione which took part in the oxidation reduction reaction in vivo inhibited the enzyme activity at a high concentration. However, it accelerated the enzyme activity at a low concentration.