Simultaneous Analysis of Several Mycotoxins in Grains by Highperformance Liquid Chromatography
Studies on Chemical Analysis of Mycotoxins Part XXII
1992 Volume 39 Issue 7 Pages 632-640
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1992 Volume 39 Issue 7 Pages 632-640
As foods are easily infected with one or more fungi, contamination by different mycotoxins may occur. High-performance liquid chromatographic (HPLC) methods were examined for separation and detection of coexistent mycotoxins. Mycotoxins were devided into two groups by amino type (BOND ELUTE NH2) mini-column chromatography, and each mycotoxin in both groups was analyzed using reversed-phase HPLC with ultraviolet and photo diode-array detectors. These results were confirmed by HPLC and normal-phase thin layer chromatography. Mycotoxins belonging to group-1 and group-2 are as follows: group-1: aflatoxin B1, B2, G1, G2, M1, M2, ochratoxin A, citrinin, patulin, sterigmatocystin and zearalenone, group-2: deoxynivalenol, nivalenol and fusarenon X. Recoveries of 11 mycotoxins of group-1 and 3 mycotoxins of group-2 spiked to rice and corn at the level of 50 ppb were 70-94% and 50-71%, respectively.
