1994 Volume 41 Issue 1 Pages 31-36
In order to recover glutaminase added to koji hydrolysate before alcohol fermentation through bioreactor, we investigated the enzyme immobilized on a magnetic support prepared from partially deacetylated chitin (PDAC), chitosan and Fe3O4. The magnetic support (MC4P) was superior in endurance to high salt concentration in the solution over a long period. The MC4P was easily recovered by magnetic force. Glutaminase (15 U) immobilized on MC4P (1g) under suitable preparation condition expressed 100% enzyme activity compared to free enzyme. Glutaminase activity immobilized on MC4P was stable in the 15% NaCl solution for 36 days.
