Quantitative Analysis of Gymnemic Acids by High Performance Liquid Chromatography

Abstract

For the quality evaluation of leaves of Gymnema sylvestre R.Br. and the extract, total gymnemic acids was determined by HPLC analysis of gymnemagenin, which was the main sapogenin obtained from a mixture of gymnemic acids by alkaline saponification followed by acid hydrolysis. It was found that total gymnemic acids content in four specimens of leaves imported from India was 3.9-4.6%, and that in seven commercial samples of leaves extracts was 3.9-9.6%.