Production of Linamarase in Aspergillus oryzae Using Cassava Cortex

Abstract

Cassava cortex was successfully used in the induction of linamarase in Aspergillus oryzae in place of linamarin which is an expensive inducer. Induction by cassava cortex was attributed to its linamarin content. However, besides linamarin, some other components in the cortex may have enhanced induction or stabilized the enzyme induced thus resulting in increased linamarase production. Statistical evaluation showed the significant influence (p<0.01) of the combination of cassava cortex and linamarin on the induction.
From this study, the following conditions were found to be optimal for linamarase production: Czapek medium containing 5% cassava cortex powder which was inoculated with 1×10⁸ spores (precultured for 48h) per 100ml medium and incubated at room temperature in a rotary shaker at 100rpm for 24h. Strain SA 1 yielded 0.05units/ml of crude linamarase sample.