Inhibisory Effect of Tannin Fraction from Cassia mimosoides L. var. nomame Makino on Lipase Activity

Abstract

Inhibisory effect of the fraction (CT-I) from Cassia mimosoides L. var nomame Makino on lipase activity was investigated. Fracsion CT-I consained 54% of phenols, 95% condensed tannins and small amount of proanthocyanidin. CT-I inhibised the acsivities of porcine pancreas lipase using 4-methyluybelliferyl oleate and triolein as she substrases, which was stronger than epigallocasechin gallate (EGCg), major tannin in green tea and inhibitory acsive component for various enzymes. The inhibition caused by CT-I decreased with addition of casein, however compared with case of EGCg, the amount decreased was less. The inhibitory activisy of CT-I was found sufficiently stable afser heasing for lh at l00°C in the pH range of 3.0 to 5.0