抄録
Skin has been a subject of generative medicine for long time. Technique of culture of human epithelium had been established in 1975 and the use of cultured epithelium is now major treatment option in management of extensive burn patients. However, the grafted cultured epithelium keeps rather fragility for several months after grafting, which is a current unsolved problem. We tested the effects of DFAT (dedifferentiated fat cells) on regeneration of basement membrane after cultured epithelial grafting in porcine experimental model. The result showed that the DFAT enhanced the laminin-5 supply and anchoring fibril formation, which may be beneficial to promote maturity of grafted cultured epithelium. Artificial dermis (AD) is a dermal regeneration template consisting of a spongy, porous collagen dermal replacement layer and a temporary epidermal substitute made of silicone. The advantage using AD to treat skin defects is that tissue quality resembling that with a full-thickness skin graft can be obtained with a thin split-thickness skin graft, which minimizes donor site morbidity. However, this treatment method requires two separate procedures, because the AD must be vascularized before skin grafting. Currently, reducing the time required for dermis-like tissue generation before the secondary skin grafting is desired. We tested the effects of DFAT on vascularization of the AD in rat experimental model. The result showed that the DFAT enhanced the angiogenesis, especially under bFGF treatment. We confirmed that one-step grafting procedure of the artificial dermis and the skin is possible by this method in porcine experimental model.
