Effects of androgens on cultured cells derived from canine anterior cruciate ligament

Hideki OHNO; Yoko KOWATARI; Masao OWAKI; Joji OHTA; Naoshi NAKAJIMA; Kazuki YOSHIOKA; Ken-ichiro MUTOH; Toshifumi OYAMADA

doi:10.2535/ofaj.89.35

Hideki OHNO, Yoko KOWATARI, Masao OWAKI, Joji OHTA, Naoshi NAKAJIMA, Kazuki YOSHIOKA, Ken-ichiro MUTOH, Toshifumi OYAMADA

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Keywords: Androgen receptor, Anterior cruciate ligament, Cell culture, Collagen, Fibroblast

JOURNAL FREE ACCESS

2012 Volume 89 Issue 2 Pages 35-38

DOI https://doi.org/10.2535/ofaj.89.35

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Abstract

Anterior cruciate ligament (ACL) fibroblasts obtained from beagle dogs were cultured in basal medium containing different concentrations of 1 to 10^-3 µM 5α-dihydrotestosterone (DHT) and in basal medium itself as a control. It was demonstrated that DHT promoted cell proliferation activity, expression of androgen receptor, and collagen synthesis in ACL fibroblasts as compared with control. These results suggest that sex hormones are involved in the sex difference seen in ACL rupture of dogs.

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