In this study, the results of our previously reported technique of quantitative analysis by using microscopic image analysis of tissue image slices to calculate the proportion of the area of the tunica media occupied by of elastic fibers was compared with Janzen et al.'s technique at the carotid bifurcation. This particularly analyzed the area of transition between the common carotid and the internal carotid, to observe the quantitative changes in elastic fiber content. The data obtained from our quantitative analysis of elastic fibers were clearly at variance with those obtained by counting the number of elastic fibers. The amount of elastic fibers in the tunica media (the elastic fiber ratio) decreased from the proximal carotid artery (the common carotid) to the bifurcation, then peaked in the internal carotid immediately after the bifurcation before declining again.
We microscopically examined the dorsal lingual surface of an old leopard (Panthera pardus). The filiform papillae on the lingual apex were dome-shaped. The connective tissue cores of the filiform papillae resembled a well in shape, while those of the fungiform papillae were not hollow. The filiform papillae on the anterior part of the lingual body were large and cylindrical in shape. The connective tissue cores of these filiform papillae consisted of large conical papillae. The filiform papillae on the central part of the lingual body were large and conical-shaped. The connective tissue cores of the filiform papillae consisted of a large main process. The vallate papillae were surrounded by grooves. The top of the connective tissue core of the vallate papilla had many ditches. These findings are peculiar to the tongue of members of the family Felidae.
We microscopically examined the dorsal lingual surface of an adult lion-tailed macaque (Macaca silenus). The tongue of the chimpanzee was about 13 cm long. Filiform papillae on the lingual apex consisted of several pointed processes. There were dome-shaped fungiform papillae scattered among the filiform papillae. The connective tissue cores of those filiform papillae consisted of processes of various size, while these of the fungiform papillae had several ditches. The vallate papilla was surrounded by a groove and pad, and were flattened-oval shaped. The connective tissue cores of the vallate papillae were covered with numerous small spines. Many foliate papillae were observed on the posterolateral regions of the tongue. After removing epithelium from the foliate papillae many processes became apparent.