Experimental Study on the Peroxidase Reacting Granules in the Blood Cells.

The 11th Report of Histochemical Study of Peroxidase

Abstract

The materials used in the experiments were the blood cells of healthy persons, animals (rabbits and guinea pigs) and human corpses, and the NAS-Benzidine (NAS-B) solution was chiefly adopted for the peroxidase stain due to its simplicity and sensitivity. The latter solution, however, does not differ essentially from the acid peroxidase stains heretofore proposed. This experimental study dealt with various inhibitors of the peroxidase reaction, postmortem changes of the blood cells, obtaining the following results:
1) The light rays exert inhibitin g influences upon the peroxidase reaction of the leukocytes in the following order: Ultraviolet, visible and infra-red rays. Therefore it can be said that the longer the wave length, the weaker becomes the inhibiting influence. However, this inhibiting influence is by no means found in the erythrocytes.
2) Methanol exerts a stronger inhibiting infl u ence upon the peroxidase reaction of the leukocytes than ethanol, however, the reaction of the erythrocytes is by no means inhibited by these alcohol, but rather is activated.
3) Excessive hydrogen peroxide solution contained in the peroxidase stain inhibits the peroxidase reaction of the leukocytes, but not that of the erythrocytes.
4) The erythrocytes are peroxidase negative under neutral or alkaline condition, while the myeloid leukocytes are positive under both acid and alkaline conditions.
5) The peroxidase reaction positive granules in the leukocytes show various colorsyellow, brown, green, blue, purple, black and other transitional colors. This is, I infer, due to the changes of the benzidine derivatives^19,43 caused by various factors such as heating, fixation and pH etc.