Abstract
1. The influence of ninh y drin on the activity of the alkaline phosphatase was examined.
2. In the inje c tion method, remarkable decrease of the activities are noticed chiefly in the central region of the liver sections aft the 30 minutes cases after the injection of the aqueous solution of ninhydrin. In the kidney sections, the decrease of the activity of the enzyme is recognized into the epithelial cells of the distal convoluted tubules in the 30 minutes cases after the injection, in the glomeruli, in the one hour case, and in the epithelial cells of the proximal convoluted tubules, in the six hours cases.
3. M the results of the method in which the sections are stained after they are exposed to the solution of the drugs directly, the activities in the liver and kidney sections disappear after they are incubated in the 1% aqueous solutions of ninhydrin for 30 minutes at 37°C.
4. As the method in which the sections are stained after they are treated with the mixture of the substrate solution and the solution of the drug, the activities of the liver and kidney sections and undemonstrable when the sections are stained after they are incubated in the mixture of the substrate solution and 5 cc of the 1% aqueous solution of ninhydrin.
We should like to record our thanks to Prof. Dr. T. Taniguchi for his helpful guidante and criticism.
