An investigation on the cells of Nucl. preopticus in Teleostei

Abstract

1. The cells of Nucl. preopticus in Anguilla japonica, Cyprinus carpio and Carassius auratus were investigated from a comparative cytological view point, using Gomori's C. H. P. and Heidenhain's iron hematoxylin staining methods.
2. With the Gomori's staining method, the cells in Anguilla japonica and in Cyprinus carpio were large and specific polymorphic in shape. The whole cell looked deep blue on account of the fine granules. With the Heidenhain's iron hematoxylin staining method, such fine granules as seen with Gomor is staining method, are not stained, but only the small granules were seen, besides they were fewer than those stained with Gomori's staining. The difference in the stainability of the granules between Gomori's staining and Heidenhai n's iron hematoxylin staining is on account of the weak stainability of the granules for iron hematoxylin, or of the presence of entirely different granules from the granules stained with both staining methods.
3. In Carassius auratus, the size of the cells and the stainability of the cytoplasm are different from the other fish. With Gomori's staining method, the fine granules are few but, on the other hand, small granules which are somewhat larger than those in the other fish are numerous.
4. On the cells of Nucl. preopticus in Anguilla japonica, the small vacuoles were seen surrounded with fine granules in the cytoplasm.