Abstract
1. Hematein shows a stron g affinity to elastic fibers at pH 6.0-8.0.
2. Affinity of hematein to elastic fibers can be augmented foll o wing addition of the mordants such as aluminum ammonium sulfate, ferric ammonium sulfate, phosphotungstic acid and ammonium molybdate.
3. Both hydrogen and ionic bonds in elastin partic i p ate in the staining of elastic fibers with hematein.
