Demonstration of Binding and Internalization of ¹²⁵I-glucagon in Hepatocytes of Intact Mouse Liver

Abstract

Distribution of labeled material in hepatocytes of intact mouse liver injected via the portal vein with ¹²⁵I-glucagon was studied. At 3 minutes after pulse injection of the labeled glucagon, the grains were observed mainly over peripheries of hepatocytes, and partially in the sinusoidal lumina and cells. At 10minutes after the injection, the grains were generally scattered over hepatocytes. At 20 minutes, the grains over hepatocyts appeared decreased in number. Thus, in hepatocytes of intact liver, labeled glucagon is internalized more rapidly than in freshly isolated hepatocytes (Barazzone et al.,1980).