Peroxidase Activity and Cell Differentiation in Developing Salivary Glands of the Rats

Abstract

Peroxidase (PO) activity-positive cells were found to develop in both the sublingual and submandibular glands of the rat on day 19 of gestation. The PO activity in the nuclear envelope (NE), endoplasmic reticulum (ER), Golgi apparatus (G) and secretory granules (SG) of these cells were enhanced day by day. However, no PO activity was detected in the parotid gland on the same day. In the parotid gland PO-positive cells were detected first on postnatal day 1. After birth the PO activity in the SG of both the sublingual and submandibular glands gradually diminished in intensity and disappeared, whereas the activity persisted in the parotid gland. From postnatal day 1 to 14, the NE and ER of the cells in the parotid and sublingual glands exhibited intense PO activity, while cells containing mucous SG appeared. The cells were identified as mucoserous acinar cells. These mucoserous cells later differentiated into different cell types: serous cells in the parotid gland and mucous cells in the sublingual gland. As the submandibular gland developed, PO activity-positive serous cells also differentiated into mucoserous cells and the activity in the G and SG disappeared. The parotid, sublingual and submandibular glands of perinatal rats have clearly showed varied growth, with the advent processes of PO activity and cell differentiation, whereas PO activity in the G concomitantly occurred with SG activity.