Descending Projections from the Paraventricular Hypothalamic Nucleus to the Spinal Cord: Anterograde Tracing Study in the Rat

Abstract

We investigated the direct projections from the paraventricular hypothalamic nucleus (PVH) to the spinal cord. When Phaseolus vulgaris leucoagglutinin (PHA-L) was injected into the PVH, labeled descending fibers were observed running bilaterally through three pathways. The first pathway ran into the dorsal longitudinal fasciculus and projected to the central gray matter, Edinger-Westphal nucleus, pedunculopontine tegmental nucleus, nucleus of the locus ceruleus and parabrachial nucleus. The second and third pathways coursed through the medial forebrain bundle, ventral tegmental area, and ventral part of the medulla oblongata. At the medulla oblongata, the second pathway curved dorsolaterally and joined Rexed's lamina V of Cl after giving many projections to the nucleus ambiguus, nucleus of the solitary tract, dorsal motor nucleus of the vagus, and a few to the area postrema. The fibers descended through lamina V until C5, and coursed through lamina I from C6 to the upper coccygeal segments. They gave collateral projections to lamina I from the cervical through the upper coccygeal segments. The third pathway coursed laterally and descended through the lateral funiculus after giving projections to the lateral reticular nucleus and the marginal layer of the spinal trigeminal nucleus. These fibers gave off many projections to the intermediolateral cell column of the thoracic cord and the sacral parasympathetic nucleus. Lamina X received many projections from the fibers of the lateral funiculus at C5through the b-segment of sacral spinal cord.
These results indicate that the PVH may integrate directly with the medullary and spinal autonomic regulatory nuclei, including the vagus complex, sympathetic intermediolateral cell column, laminae I and X, and sacral parasympathetic nucleus.