Development and Application of an Enzymatic Indirect Method for Simultaneous Analysis of 3-MCPD Esters, 2-MCPD Esters and Glycidyl Esters

Abstract

The concerns about potential health risks associated with fatty acid esters of 3-chloro-1, 2-propanediol (3-MCPDEs), 2-chloro-1, 3-propanediol (2-MCPDEs) and glycidol (GEs) in edible oils and fats have led to development of various methods for quantitative determinations of these com pounds in various countries. An enzymatic indirect method for simultaneous determinations of 3-MCP DEs, 2-MCPDEs and GEs were developed based on Candida cylindracea lipase hydrolysis of esters. JOCS conducted a collaborative study with 13 participant laboratories, and obtained results with good reproducibility. In 2016, the enzymatic indirect method for analysis of oils and fats except for fish oils was registered in “The JOCS Standard Methods for the Analysis of Fats, Oils and Related Materials” as 2.4.14_-2016. Then, the enzymatic indirect method was successfully applied to the analyses of fish oils and foods containing oils and fats. The method for analysis of fish oils was registered in “The JOCS Stan dard Methods for the Analysis of Fats, Oils and Related Materials” as R7_-2017.