2023 Volume 23 Issue 11 Pages 549-558
Staphylococcus aureus (S. aureus) increases at inflammation site of atopic dermatitis, and aggravates the inflammation. On the other hands, Staphylococcus epidermidis (S. epidermidis) is a common microbiota on healthy skin and has several beneficial functions such as suppression of S. aureus growth. This study evaluated antibacterial activity of many fatty acids against S. aureus and S. epidermidis. The results showed that 6-cis-C16:1, 7-cis-C16:1, and 9-cis-C16:1 possessed selective antibacterial activity that strongly suppressed S. aureus growth and weakly suppressed S. epidermidis growth, and the function may be beneficial for decrease inflammation at atopic dermatitis skin. The 6-cis-C16:1 included in skin lipids is rarely observed in natural oils, we thus investigated microbial-mediated production of 7-cis-C16:1 and 9-cis-C16:1. Aeromonas hydrophila and Acinetobacter sp. converted 9-cis-C18:1 to 7-cis-C16:1 when the strains were cultivated with vegetable oils. Genetic modified Saccharomyces cerevisiae produced triacylglycerols including high content 9-cis-C16:1, and the following purification processes resulted in 98% 9-cis-C16:1. Bifidobacterium sp. JCM 7042 produced 7-cis-C16:1 inside the cells.
