抄録
Effect of a saline bitter spring on the capillary formation in the granulation tissue was studied with small collodion pipes (length: 8mm, inner diameter: 1.5mm, wall thickness: 0.3-0.5mm) which were embeded into the abdominal subcutaneous tissues of 9-12 rats, four of them in each rat.
Rats were bathed in a saline spring water at 38°C for 10 minutes daily during 3 weeks since the 3rd day after collodion pipe embedding. The pipes of about one-third of the whole rats were taken out every week to study histologically.
Similar experiments were carried out with a series of baths in a control plain water, in an aged thermal water, or in a synthetic saline water, and also with intravenous injection of sodium chondroitin sulfate or heparin solution.
Following results were obtained:
1. Capillary formation of granulation tissue in the collodion pipes was increased by a series of thermal baths in the saline bitter water.
2. Cells of various kinds, such as histiocytes, mononucleares and fibroblasts were more abundantly observed in the granulation tissue grown in the pipes after a series of baths in the saline bitter spring as compared with the results with the control plain water baths.
3. Bloodways, which were surrounded by these cells and differentiated later partially into capillaries, were more frequently observed in the thermal water bathed rats than in the control groups which were bathed in plain water or aged thermal water.
4. Cicatrication of granulation tissue in the collodion pipes was considerably refreshed by bitter spring baths for 3 weeks. Namely scar tissues were filled vigorously with blood and their shrinking was seemingly delayed after a series of baths in the bitter spring as compared with the results with the control plain water bath group.
5. Many lymphocytes were observed in collodion pipes of rats, to which 0.1-0.15ml of 1% sodium chondroitin sulfate or 0.2% heparin solution was administered intravenously daily for 3 weeks, but capillary formation was not so much increased as in the collodion pipes of rats bathed in a saline bitter spring.
The similarities between the granulation tissues in the collodion pipes of the rats that were bathed in a synthetic saline bitter water and of the rats, to which were given intravenously sodium chondroitin sulfate or heparin solution, were mentioned referring to the action of sulfate ions in the connective tissues.
